Rat soluble E-selectin,sE-selectin ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Bring all reagents to room temperature (18-25°C) before use for 30min. -Prepare fresh standard for each assay. Use within 4 hours and discard after use. -Centrifuge the sample again after thawing before the assay.	- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. -Once reagents have been added to the well strips, DO NOT let the strips DRY at any time during the assay. -TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light. -Stop Solution should be added to the plate in the same order as the TMB Substrate.

Upstream tips
Bring all reagents to room temperature (18-25°C) before use for 30min. -Prepare fresh standard for each assay. Use within 4 hours and discard after use. -Centrifuge the sample again after thawing before the assay.
Protocol tips
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. -Once reagents have been added to the well strips, DO NOT let the strips DRY at any time during the assay. -TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light. -Stop Solution should be added to the plate in the same order as the TMB Substrate.

Publication protocol

Serum PEDF was quantified (pg/ml) using a commercially available enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Cusabio Biotech, Rat pigment epithelium-derived factor ELISA kit, Cat. no: CSB-E08819r, Hubei Province, China).



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Manufacturer protocol

Download the product protocol from Cusabio for Rat soluble E-selectin,sE-selectin ELISA Kit below.

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