Rat Endothelin 1 PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

The levels of Abeta1–40 and Abeta1–42 were examined by ELISA according to the manufacturer’s protocols (Wako, Japan). One hundred microliters of cell lysate (1:10 dilution) from each sample was assayed in the study, with equal volume of standard solution as internal control. The data were presented as Abeta peptides (pmol) per gram (g) of total cell lysate protein (pmol/g), and the tunicamycin treated groups were compared with control by one-way ANOVA (p < 0.05 was conceived as statistically significant).

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Manufacturer protocol

Download the product protocol from BosterBio for Rat Endothelin 1 PicoKine™ ELISA Kit below.

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