17β-Estradiol ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.	-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Upstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.
Protocol tips
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Publication protocol

Total carbohydrate in mouse and rat milk was determined by colorimetric assay (K645 BioVision). Total protein was determined using the bicinchoninic assay and milk fat was determined as described (Forcato et al., 2005). For assays of macronutrients, there were n = 4 mice in each group and n = 6 rats. Mouse milk BDNF was determined by ELISA (OKEH00022, Aviva Systems Biology) with n = 8 in each group.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for 17β-Estradiol ELISA kit below.

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