17 beta Estradiol ELISA Kit (ab108667)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Serum ALT (98-24010-US), AST (98-24016-US), TC (98-24005-US), glucose (98-24009-US), and TG (98-24019-US) concentrations were measured using an autoanalyzer (IDEXX VetTest® Chemistry Analyzer, IDEXX Laboratories, Inc., Westbrook, ME, USA) according to the manufacturer's instructions. The VetTest apparatus requires 70 ul volumes of serum for all parameters. The different biochemical tests are available as dry slides that include all necessary reagents. Serum estradiol level was determined using Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab108667, Abcam, Cambridge, UK) according to the manufacturer's instructions.

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Manufacturer protocol

Download the product protocol from Abcam for 17 beta Estradiol ELISA Kit (ab108667) below.

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