Rat / Mouse FGF-21 ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Maintain the unopened kit at 2-8 C.	-Vigorous washing and complete removal of all liquid by aspiration at the end of each washing step is very important to obtain low background values. -Dilute the reagents immediately before use.	-Do not store diluted solutions. - Maintain the BDNF Standard, at 2-8 C for up to 30 days after reconstitution.

Upstream tips
-Maintain the unopened kit at 2-8 C.
Protocol tips
-Vigorous washing and complete removal of all liquid by aspiration at the end of each washing step is very important to obtain low background values. -Dilute the reagents immediately before use.
Downstream tips
-Do not store diluted solutions. - Maintain the BDNF Standard, at 2-8 C for up to 30 days after reconstitution.

Publication protocol

Insulin and fibroblast growth factor-21 (FGF-21) in serum were analyzed by commercially available kits as per the manufacturer’s instructions [rat ultrasensitive insulin (ALPCO, Salem, NH); rat/ mouse FGF-21 (Millipore, Billerica, MA)].

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Manufacturer protocol

Download the product protocol from Merck Millipore for Rat / Mouse FGF-21 ELISA Kit below.

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