Rat Gastrin 1 ELISA Kit (ab133049)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Just before stomach removal, blood was collected from the abdominal aorta. The heparinized blood was centrifuged at 3,000×g for 10 min and the plasma was kept at –20°C until analysis. The plasma level of gastrin was determined using a commercially available enzyme-linked immunosorbent assay kit (Abcam, Cambridge, UK).

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Manufacturer protocol

Download the product protocol from Abcam for Rat Gastrin 1 ELISA Kit (ab133049) below.

Download manufacturer protocol

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