Human GDNF DuoSet ELISA

Protocol tips

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	Homogenized tissues at 100 mg ml-1 in 1 × phosphate buffered saline+0.5% Triton X-100+protease inhibitor cocktail (Roche, Indianapolis, IN, USA) were centrifuged at 4 °C at 4000 rpm for 5 min. Supernatant was collected and stored at −80 °C. DuoSet ELISA kit determined GDNF concentration (DY212, R&D systems) according to the manufacturer's instructions. Samples were run with biological (n>4 per group) and technical (n=3) replicates.

Protocol tips

Homogenized tissues at 100 mg ml-1 in 1 × phosphate buffered saline+0.5% Triton X-100+protease inhibitor cocktail (Roche, Indianapolis, IN, USA) were centrifuged at 4 °C at 4000 rpm for 5 min. Supernatant was collected and stored at −80 °C. DuoSet ELISA kit determined GDNF concentration (DY212, R&D systems) according to the manufacturer's instructions. Samples were run with biological (n>4 per group) and technical (n=3) replicates.

Publication protocol

Homogenized tissues at 100 mg ml-1 in 1 × phosphate buffered saline+0.5% Triton X-100+protease inhibitor cocktail (Roche, Indianapolis, IN, USA) were centrifuged at 4 °C at 4000 rpm for 5 min. Supernatant was collected and stored at −80 °C. DuoSet ELISA kit determined GDNF concentration (DY212, R&D systems) according to the manufacturer's instructions. Samples were run with biological (n>4 per group) and technical (n=3) replicates.

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