HO-1 (rat), ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.	-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Upstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.
Protocol tips
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Publication protocol

The concentrations of rat MPO proteins in the BALF samples in all of the examinations were measured by ELISA kits, HK105 (Hycult Biotech, The Netherlands). The concentrations of CINC-1 in the BALF were measured by ELISA kits, #RCN100 (R& D Systems, Minneapolis, MN), and the concentrations of HO-1 in the NiO, TiO2 (P90), TiO2 (Rutile), CeO2, and ZnO exposure examinations were measured by an ELISA kit, ADI-EKS-810A (Enzo Life Sciences, Farmingdale, NY). The activity of LDH in the NiO, TiO2 (Rutile), CeO2, and ZnO exposure examinations was measured by a Cytotoxicity Detection KitPLUS (LDH) (Roche Diagnostics GmbH, Mannheim, Germany). All measurements were performed according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for HO-1 (rat), ELISA kit below.

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