HSP70 High Sensitivity ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.	-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Upstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.
Protocol tips
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Publication protocol

HSP70 plasma and liver concentrations were measured using the rat HSP70 enzyme-linked immunosorbent assay (ELISA) Kit (Enzo Life Sciences, Inc., NY; ADI-EKS-715). The smallest concentration of a HSP70 that could be reliably measured by selected analytical Kit was 90 pg/ml, with detection range 0.20–12.5 ng/ml; ADI-EKS-700B, minimum sensitivity 200 pg/ml (detection range 780–50,000 pg/ml) according to the manufacturer’s instructions. HSP90 plasma and liver concentrations were measured using the rat HSP90 ELISA (Wuhan USCN Business Co., Ltd. Product no SEA823Ra). The smallest concentration of a HSP90 that could be reliably measured by selected analytical Kit was 0.055 ng/ml, with detection range 0.156-10 ng/ml. Each experiment was performed in duplicate.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for HSP70 High Sensitivity ELISA kit below.

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