HSP70 ELISA Kit (ab133060)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Homogenates (2 mg/mL total protein) were analyzed using a multi-plexed immuno assay for a rat cytokine/chemokine panel (RECYMAG65K27PMX; EMD Millipore, MA). The same homogenates were also analyzed by enzyme-linked immunosorbent assays (ELISA) for the following cardiac injury markers: heart-type fatty acid binding protein (H-FABP) (MBS725463; MyBioSource Inc., San Diego, CA), cardiac troponin I (cTnI) (LS-F23616; LSBio Inc., Seattle, WA), N-terminal pro b-type natriuretic peptide (NT-proBNP) (LS-F23593; LSBio Inc.), and tumor necrosis factor alpha (TNF-α) (AB46070; Abcam, Cambridge, MA) and heat shock protein 70 (HSP70) (AB133060; Abcam). All assays were performed according to manufacturers’ protocols and were read on a spectrophotometric plate reader (Spectra Max M5, Molecular Devices, Sunnyvale, CA).

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Manufacturer protocol

Download the product protocol from Abcam for HSP70 ELISA Kit (ab133060) below.

Download manufacturer protocol

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