Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

To measure the circulating levels of IGF-I expression in animal serum, serum was collected from the tails of rats at 8 weeks post-GH therapy. The ELISA was performed per the commercial IGF-I rat/mouse quantikine ELISA kit instructions (MG100, R&D Systems, USA). In brief, 50ul of serum was mixed with 50uL of assay diluent in a 96-well plate and incubated for 2 hours at room temperature. The supernatant was aspirated, and the wells were serially washed with wash buffer. A substrate solution was then added to each reaction and incubated at room temperature while in the dark for 30 min. Lastly, the reaction was terminated by adding 100ul of stop solution. Utilizing OD 570 as a reference, absorbance at 450 nm was recorded using a TECAN infinite 200 PRO (Tecan, USA).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Rat - IGF-I using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit from R&D Systems.

Manufacturer protocol

Download the product protocol from R&D Systems for Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Rat - IGF-I using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit from R&D Systems. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.