Rat IGF-1 ELISA Kit (ab213902)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

The primary rat cortical neurons in 60-mm dishes were cultured in serum-free base medium with the additional treatment according to the experimental groups described above. After 30 h, the supernatant was collected and centrifuged at 135 × g for 5 min at room temperature. The concentrations of IGF-1 or IGF-2 in the supernatant were quantified using a rat IGF-1 ELISA kit or a rat IGF-2 ELISA kit (cat. nos. ab213902 and ab213903; Abcam), respectively, according to the manufacturer's protocols. The optical density at 450 nm was measured using a microplate reader (Epoch2; Bio-Tek Instruments, Inc., Winooski, VT, USA).

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Manufacturer protocol

Download the product protocol from Abcam for Rat IGF-1 ELISA Kit (ab213902) below.

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