ZR RNA MiniPrepTM kit

RNA isolation / purification Cells - primary human endothelial cells

Experiment
RNA isolation / purification Cells - primary human endothelial cells
Product
ZR RNA MiniPrepTM kit from Zymo Research
Manufacturer
Zymo Research

Protocol tips

Upstream tips
- The kit is designed for efficient isolation of total RNA from 1x10^2to 1x10^7 cells.

Publication protocol

HUVECs were seeded in 6 well plate at 1.2 ×106 cells /well overnight, then treated with LPS (10 ng/mL) with or without Crocetin (1, 5, 10 ng/mL) for 24hr. After that, total RNA was isolated by ZR RNA MiniPrep (Zymo Research) and reverse transcripted into cDNA by High capacity cDNA reverse transcription kit (Applied Biosystems) in accordance with manufacturer's instructions.

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Reviews

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Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - primary human endothelial cells using ZR RNA MiniPrepTM kit from Zymo Research.

Paper title
Crocetin Inhibits Lipopolysaccharide-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells.
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Manufacturer protocol

Download the product protocol from Zymo Research for ZR RNA MiniPrepTM kit below.

Download PDF Download manufacturer protocol

Videos

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