Rat IL-1 beta ELISA Kit (ab100768)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

Homogenates from the hippocampus were centrifuged at 12,000 rpm for 10 min at 4°C as described previously (14), and the supernatants were collected. The concentrations of IL-1β and TNF-1α were measured with an ELISA kit (cat. no. ab100768 and ab46070; Abcam), using 100 µl of protein for detection according to the manufacturer's instructions. Each experimental condition was tested in three different wells and measured in duplicate.

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Manufacturer protocol

Download the product protocol from Abcam for Rat IL-1 beta ELISA Kit (ab100768) below.

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