Rat IL-6 PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Using the fully automatic biochemical analyzer
TBA-40FR (Toshiba, Minato, Tokyo, Japan), the
contents of creatinine (Cre) and blood urea nitrogen (BUN) were measured by reagent kits according to the manufacturer’s protocols (BioSino, Beijing,China). The levels of tumor necrosis factor-α
(TNF-α) and interleukin-6 (IL-6) were detected
using enzyme-linked immunosorbent assay kits
specific for rats (Boster, Wuhan, China).

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Manufacturer protocol

Download the product protocol from BosterBio for Rat IL-6 PicoKine™ ELISA Kit below.

Download manufacturer protocol

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