Leptin (rat), ELISA kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.	-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Upstream tips
-All components of this kit except the standard are stable at 4˚C. The standard must be stored at or below -20˚C. -Bring all reagents except the standard and assay buffer to room temperature for at least 30 minutes prior to opening.
Protocol tips
-Diluted standards and samples should be kept on ice and used within 60 minutes of preparation for optimal performance. If ice is not available, diluted standards and samples should be used within 20 minutes of preparation. -Prior to the addition of antibody, conjugate or substrate, ensure there is no residual wash buffer in the wells. Remaining wash buffer may cause variation in assay results.

Publication protocol

At 90 days of life, leptin plasma levels were determined using an ELISA kit (Enzo® Life Sciences, Plymouth Meeting, PA, USA). The leptin intra- and interassay variation coefficients were 5.9 and 7.2%, respectively.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for Leptin (rat), ELISA kit below.

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