Rat MCP1 ELISA Kit (CCL2) (ab100778)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

As described above, livers, epididymal fat depots, and jejunal segments were excised from the animals immediately after sacrifice and stored at − 80 °C until analysis. Tissue protein concentrations of the three tissues were first determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, MA, USA) method according to manufacturer’s instruction. TNF-α and MCP-1 levels were subsequently determined in tissue homogenates with enzyme-linked immunosorbent assay (ELISA) kits (AB100785 and AB100778; Abcam, Cambridge, UK) according to manufacturer’s instructions. Absorbance was read using a Spectramax 340PC384 microplate reader at 450 nm (Molecular Devices, CA, USA).

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Manufacturer protocol

Download the product protocol from Abcam for Rat MCP1 ELISA Kit (CCL2) (ab100778) below.

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