Rat Lipocalin-2 ELISA Kit (NGAL) (ab119602)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Serum neutrophil gelatinase-associated lipocalin (NGAL) levels were measured post hoc by ELISA (Abcam, Cambridge, MA) according to the manufacturer’s directions.

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Discussion

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Manufacturer protocol

Download the product protocol from Abcam for Rat Lipocalin-2 ELISA Kit (NGAL) (ab119602) below.

Download manufacturer protocol

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