Rat NGF/NGF Beta PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Five animals in the CytoCon group were sacrificed at 3 days after SCI, then five animals each from the CytohUCB group and CytoCon group were sacrificed at 7, 14, 21 and 28 days after SCI. Following sacrifice, the spinal cord (T7–T12) was extracted and homogenized with a cocktail of proteases inhibitors (Sigma, USA) in RIPA buffer (Millipore, USA), then centrifuged at 10,000 × g and 4℃. Supernatants were harvested and quantitative analysis of nerve growth factor (NGF), brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) was conducted using an ELISA kit (Boster Biological Technology, China).

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Manufacturer protocol

Download the product protocol from BosterBio for Rat NGF/NGF Beta PicoKine™ ELISA Kit below.

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