Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

Serum cGMP (IBL RE29075), H-FABP (Hycult Biotech, ELISA HK414, Uden, The Netherlands), BNP (Biotrend RK011-14), and osteopontin (Quantikine ELISA, MOST00, R&D Systems) were quantified according to the assay manual provided by the manufacturers. Myocardial cGMP was analyzed by a commercial cGMP EIA kit (Cayman Chemical Co.) according to the manufacturer’s protocol.

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Manufacturer protocol

Download the product protocol from R&D Systems for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit below.

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