Rat prolactin,PRL ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Bring all reagents to room temperature (18-25°C) before use for 30min. -Prepare fresh standard for each assay. Use within 4 hours and discard after use. -Centrifuge the sample again after thawing before the assay.	- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. -Once reagents have been added to the well strips, DO NOT let the strips DRY at any time during the assay. -TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light. -Stop Solution should be added to the plate in the same order as the TMB Substrate.

Upstream tips
Bring all reagents to room temperature (18-25°C) before use for 30min. -Prepare fresh standard for each assay. Use within 4 hours and discard after use. -Centrifuge the sample again after thawing before the assay.
Protocol tips
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. -Once reagents have been added to the well strips, DO NOT let the strips DRY at any time during the assay. -TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light. -Stop Solution should be added to the plate in the same order as the TMB Substrate.

Publication protocol

Serum concentrations of corticosterone were analyzed by radioimmunoassay after dichloromethane extraction as described previously (Jezova et al., 1994). The intra-assay and interassay coefficient of variation values were 6% and 8%, respectively, and the sensitivity of the assay was 0.5 µg/100 ml of plasma. The concentrations of free triiodothyronine and prolactin in serum were measured using commercially available enzyme-linked immunoassay (ELISA) kits (rat-free triiodothyronine ELISA kit and Prolactin rat ELISA; Cusabio Technology, Houston, TX, respectively). The intra- and inter-assay precision of the kits (coefficient of variation %) were less than 15%. The sensitivity and detection range reported by the manufacturer were 0.125 and 0.125–50 ng/ml for prolactin and 0.38 and 2–32 pmol/liter for triiodothyronine, respectively.

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Manufacturer protocol

Download the product protocol from Cusabio for Rat prolactin,PRL ELISA Kit below.

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