Human SCF ELISA Kit (ab100636)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Blood samples (2 ml) were collected from the rats prior to sacrifice. The concentrations of SCF in the plasma was determined using an ELISA kit (Abcam, Cambridge, UK) according to manufacturer's protocol.

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Manufacturer protocol

Download the product protocol from Abcam for Human SCF ELISA Kit (ab100636) below.

Download manufacturer protocol

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