Rat SCF ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.	-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.	-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Upstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.
Protocol tips
-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.
Downstream tips
-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Publication protocol

Peritoneal MCs and macrophages were harvested by peritoneal lavage of control and dexamethasone-treated rats anesthetized with ketamine, 40 mg/kg, and atropine, 5 mg/kg im (4). Before the lavage, a catheter was placed in the jugular vein to obtain a blood sample for determination of plasma concentration of stem cell factor (SCF, c-kit ligand) using an ELISA kit (Ray Biotech, Norcross, GA), after which the animals were killed with 150 mg/kg pentobarbital sodium intravenously. After lavage, MCs were separated from macrophages by differential centrifugation using a Percoll solution. MCs isolated essentially had no contamination with macrophages. The cells were counted using a hemocytometer.

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Manufacturer protocol

Download the product protocol from Raybiotech for Rat SCF ELISA below.

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