ROS-ID® Total ROS detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Cells were treated with carboxy-H2DCFDA at a final concentration of 25μM in darkness at 37°C for 30 min.

Protocol tips
- Cells were treated with carboxy-H2DCFDA at a final concentration of 25μM in darkness at 37°C for 30 min.

Publication protocol

The effect of OEA on intracellular ROS levels was tested using the total ROS detection kit according to the manufacturer’s instructions (Enzo Life Sciences Inc., Farmingdale, NY, USA) as previously described [13]. Briefly, following drug treatment, the HUVEC cells were harvested and washed with 1 × washing buffer, and then the cells were incubated with 100 μl of 5-(and-6)-carboxy-2, 7-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) (25 μM final concentration) in darkness at 37°C for 30 min. Cellular DCF fluorescence intensity was determined through microplate reader with excitation wavelength of 495 nm and emission wavelength of 529 nm. The ROS level was expressed as a percentage of the control.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - human umbelical vein endothelial cells (HUVEC) using ROS-ID® Total ROS detection kit from Enzo Life Sciences.

Manufacturer protocol

Download the product protocol from Enzo Life Sciences for ROS-ID® Total ROS detection kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - human umbelical vein endothelial cells (HUVEC) using ROS-ID® Total ROS detection kit from Enzo Life Sciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.