Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.	-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.	-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Upstream tips
-The entire kit may be stored at -20°C for up to 1 year from the date of shipment. -Avoid repeated freeze-thaw cycles. -The kit may be stored at 4°C for up to 6 months. -Bring all reagents and samples to room temperature (18 - 25ºC) before use.
Protocol tips
-Levels of Dkk-1 may vary between different samples. Optimal dilution factors for each sample must be determined by the investigator. -A standard curve must be run with each assay. -Keep substrate solution protected from light. -Read immediately after adding STOP solution.
Downstream tips
-Store your standard at <-70ºC after reconstitution, others at 4ºC.

Publication protocol

The levels of LDH (Rat LDH ELISA kit; cat. no. E-0672; Meilian Shengwu), NO (Total Nitric Oxide and Nitrate/Nitrite Parameter assay kit; cat. no. KGE001; R&D Systems), MDA (MDA assay kit; cat. no. ab118970; Abcam), and SOD (Human/Mouse/Rat Total SOD2/Mn-SOD DuoSet IC ELISA kit; cat. no. DYC3419-2; R&D Systems) from endothelial cell medium were quantified by ELISA assay according to the manufacturer's protocol.

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Manufacturer protocol

Download the product protocol from R&D Systems for Human/Mouse/RatTotal SOD2/Mn-SOD DuoSet IC ELISA below.

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