Rat VEGF Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

HIF1α levels in retinal homogenates were assayed using commercially-available rat enzyme-linked immunoassay (ELISA) kits from MyBioSource (San Diego, CA, USA). VEGF sVEGFR-1, and IGF-I levels in retinal homogenates were assayed using commercially-available quantikine ELISA kits from R & D Systems (Minneapolis, MN, USA). All assays were conducted according to the manufacturer’s protocol. All data were standardized using total cellular protein levels.

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Manufacturer protocol

Download the product protocol from R&D Systems for Rat VEGF Quantikine ELISA Kit below.

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