Rat VEGF PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Levels of VEGF, soluble fms-like tyrosine kinase-1 (sFLT-1), IL-15, and IL-18 of uDCs were analyzed by ELISA. The sensitivity of the VEGF and IL-18 ELISA kits was <1.0 pg/ml, and none of the samples had a cytokine level of >1000 or <16.9 pg/ml. There was no cross-reactivity of VEGF and IL-18 with other cytokines. The sensitivity of Flt-1 ELISA kits was 0.41 ng/ml, the interassay coefficients of variation (CV%) were <12%, and the intra-assay CV% was <10%. None of the samples had a cytokine level of >100 or <0.41 pg/ml.

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Manufacturer protocol

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