Mouse Adiponectin ELISA Kit (ab108785)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use. -Make sure the heat block/water bath and microplate reader are switched on before starting the experiment.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use. -Make sure the heat block/water bath and microplate reader are switched on before starting the experiment.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Serum, culture media and protein lysates samples were analyzed for adiponectin and leptin using an adiponectin mouse ELISA kit (ab108785, Abcam) and leptin mouse ELISA kit (KMC2281, Invitrogen).

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Manufacturer protocol

Download the product protocol from Abcam for Mouse Adiponectin ELISA Kit (ab108785) below.

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