ANGPTL3 (mouse/rat) Dual ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Plate and reagents should be at room temperature before use. -Do not expose reagents to temperatures greater than 25°C.	- Prepare just the appropriate amount of the buffers necessary for the assay. -Avoid freeze/thaw cycles. -Keep TMB Substrate Solution (TMB) protected from light.	-The diluted STREP-HRP is not stable and cannot be stored. -The reconstituted standard is aliquoted and stored at -20°C.

Upstream tips
-Plate and reagents should be at room temperature before use. -Do not expose reagents to temperatures greater than 25°C.
Protocol tips
- Prepare just the appropriate amount of the buffers necessary for the assay. -Avoid freeze/thaw cycles. -Keep TMB Substrate Solution (TMB) protected from light.
Downstream tips
-The diluted STREP-HRP is not stable and cannot be stored. -The reconstituted standard is aliquoted and stored at -20°C.

Publication protocol

After treatment, cell culture supernatant was collected and centrifuged at 1000 g for 20 min to remove particulates and assayed for Angptl3 protein secreted by podocytes by the ANGPTL3 (mouse) enzyme-linked immunosorbent assay (ELISA) Kit (AdipoGen, Liestal, Switzerland) according to the manufacturer’s protocol.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - Angiopoietin-Like 3 (AngptL3) using ANGPTL3 (mouse/rat) Dual ELISA Kit from AdipoGen Lifesciences.

Manufacturer protocol

Download the product protocol from AdipoGen Lifesciences for ANGPTL3 (mouse/rat) Dual ELISA Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - Angiopoietin-Like 3 (AngptL3) using ANGPTL3 (mouse/rat) Dual ELISA Kit from AdipoGen Lifesciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.