Mouse BMP2 ELISA Kit (ab119582)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

The concentration of vascular endothelial growth factor (VEGF; cat. no. MMV00; Bio-Rad Laboratories, Inc.), BMP-2R (MAB3552; Bio-Rad Laboratories, Inc.), receptor activator of NF-κB ligand (RANKL; cat. no, Hu7596RY; Huiying, Shanghai, China) and macrophage colony-stimulating factor (M-CSF; cat. no. KHG00112; Thermo Fisher Scientific, Inc.) In the synovial cells of experimental mice were analyzed using ELISA. Synovial cells were harvested and homogenized in an Instant One ELISA Cell Lysis Buffer (Invitrogen; Thermo Fisher Scientific, Inc.). The synovial cells were subsequently separated by centrifugation at 6,000 × g for 10 min at room temperature and the homogenate was quantified according to a previously described method (39). The levels of BMP-2R (cat. no. FK-R0025; Huiying), M-CSF (cat. no. KHG00112; Thermo Fisher Scientific, Inc.) and RANKL (cat. no, Hu7596RY; Huiying) were measured using ELISA according to the manufacturers' protocols. Levels of BMP-2 (cat. no. ab119582), IL-6 (cat. no. ab100712) and IL-10 (cat. no. ab100765) were assessed using ELISA kits obtained from Abcam (Cambridge, UK) according to the manufacturers' protocol.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse BMP2 ELISA Kit (ab119582) below.

Download manufacturer protocol

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