BMP-2 Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

The investigated markers were detected in the supernatants collected at the last day of the differentiation process, the 18th day of osteogenic stimulation and the 12th day of adipogenic stimulation. The presence of osteopontin (OPN) was determined using Mouse/Rat Osteopontin Quantikine ELISA Kit (R&D Systems, Minneapolis, USA), whereas osteocalcin (OCN) was assessed with the Mouse Gla-Osteocalcin High Sensitive EIA Kit (Takara, Otsu, Japan). The level of bone morphogenetic protein (BMP) was investigated with the Mouse BMP-2 Quantikine ELISA Kit (R&D Systems, Minneapolis, USA). All procedures were performed according to the manufacturer's protocol. The amount of detected proteins was expressed as ng/mL of supernatant.

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Manufacturer protocol

Download the product protocol from R&D Systems for BMP-2 Quantikine ELISA Kit below.

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