Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Serum of mice was respectively collected 3 days and 7 days after radiation, as well as the normal mice. The collected each sample was detected with an enzyme-linked immunosorbent assay (ELISA) kit for CRP concentrations. We did a pre-test to determine the optimal sample diluted concentration, then we added 100 ul diluted sample per well to measure CRP level according to the manufacturer’s instructions (Boster, China). Each sample was run in duplicate.

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Manufacturer protocol

Download the product protocol from BosterBio for Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit below.

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