FITC Annexin V Apoptosis Detection Kit I (RUO)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

Flow cytometry was performed as described before [15]. Briefly, the HepG2 cells were washed with cold PBS 2 times and then were resuspended in binding buffer (10 mM Hepes/NaOH (pH 7.4), 0.14 M NaCl, and 2.5 mM CaCl2), and FITC Annexin V and PI were added. After incubation at room temperature for 15 min in the dark, flow cytometry was analyzed. Annexin V-FITC and PI double staining were regarded as late apoptotic or necrotic cells.

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Manufacturer protocol

Download the product protocol from BD Biosciences for FITC Annexin V Apoptosis Detection Kit I (RUO) below.

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