Mouse Chitinase 3-like 1 Quantikine ELISA Kit

ELISA Mouse - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Experiment
ELISA Mouse - Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40
Product
Mouse Chitinase 3-like 1 Quantikine ELISA Kit from R&D Systems
Manufacturer
R&D Systems

Protocol tips

Upstream tips
-Store the unopened kit at 2-8 °C.
-Bring all reagents to room temperature before use.
-Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming.
-Substrate Solution should remain colorless until added to the plate.
-Keep Substrate solution protected from light.
-Stop Solution should be added to the plate in the same order as the Substrate Solution.
-Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with
the Substrate Solution.

Publication protocol

TGF-β1, MMP-9, VEGF, CHI3L1 and LCN2 levels were measured in lysates and sera using enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (TGF-β1: Mouse uncoated ELISA Kit, Thermo Fisher Scientific; MMP-9, VEGF, CHI3L1 and LCN2: Mouse Quantikine ELISA Kit, Biotechne, Minneapolis, MN, USA). A microplate reader was used to measure the absorbance at 450 nm and 550 nm. Readings at 550 nm were subtracted from those at 450 nm for correction purposes. A standard curve from recombinant mouse protein was constructed according to the manufacturer’s instructions to determine protein levels from the corrected optical densities using Deltasoft.

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Papers

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Manufacturer protocol

Download the product protocol from R&D Systems for Mouse Chitinase 3-like 1 Quantikine ELISA Kit below.

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