Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

The level of IL-11, IL11RA, MMP-2, and TIMP-1 in equal volumes of cell culture medium were quantified using the following kits: Human IL-11 Quantikine ELISA kit (D1100, R&D Systems), Human IL11RA ELISA kit (LSF8919, Lifespan Biosciences), Total MMP-2 Quantikine ELISA kit (MMP200, R&D Systems), Human TIMP-1 Quantikine ELISA kit (DTM100, R&D Systems). Mouse plasma level of CRP, IFNγ, TGFβ1, and TNF were measured using the following kits: CRP Quantikine ELISA kit (ab157712, Abcam), and Mouse IFNγ ELISA kit (ab100689, Abcam), Mouse TNF ELISA kit (ab208348, Abcam), and Mouse TGFβ1 ELISA kit (ab119557, Abcam)

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Manufacturer protocol

Download the product protocol from Abcam for Mouse C Reactive Protein ELISA Kit (PTX1) (ab157712) below.

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