Mouse Dkk-1 ELISA Kit

ELISA Mouse - Dkk-1

Experiment
ELISA Mouse - Dkk-1
Product
Mouse Dkk-1 ELISA Kit from Sigma-Aldrich
Manufacturer
Sigma-Aldrich

Protocol tips

Upstream tips
-Bring all reagents and samples to room temperature (18–25 °C)
before use.
Protocol tips
-Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
-Read at 450 nm immediately after adding STOP solution.

Publication protocol

The ELISA kit Quantikine Human DKK1 Immunoassay (R&D Systems, Minneapolis, Minnesota, USA) was used to determine the concentrations of DKK1 in the culture media. The optical density of each well was determined with a microplate reader (Infinite M200Pro, Tecan, Salzburg, Austria). A standard curve and the concentrations of DKK1 in the samples were obtained by reducing the data using a four-parameter logistic curve fit.

Full paper   Login or join for free to view the full paper.

Reviews

Mouse Dkk-1 ELISA Kit from Sigma-Aldrich has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - Dkk-1 using Mouse Dkk-1 ELISA Kit from Sigma-Aldrich.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Mouse Dkk-1 ELISA Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - Dkk-1 using Mouse Dkk-1 ELISA Kit from Sigma-Aldrich. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms