Endothelin 1 ELISA Kit (ab133030)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

For comparison of plasma ET-1 between groups, blood samples (about 1ml) were drawn at the time of sacrifice from the left ventricle under general anesthesia using ketamine/xylazine/normal saline cocktail (1ml:0.5ml:8.5ml, 1ml/100g B.W.). Plasma ET-1 level was evaluated using ELISA kits (ab133030, Abcam, Cambridge, UK) according to the manufacturer's instructions.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - Endothelin 1 using Endothelin 1 ELISA Kit (ab133030) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for Endothelin 1 ELISA Kit (ab133030) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - Endothelin 1 using Endothelin 1 ELISA Kit (ab133030) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.