Endothelin 1 ELISA Kit (ab133030)

ELISA Mouse - Endothelin 1

Experiment
ELISA Mouse - Endothelin 1
Product
Endothelin 1 ELISA Kit (ab133030) from Abcam
Manufacturer
Abcam

Protocol tips

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components.
-Ensure plates are properly sealed or covered during incubation steps.
-Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and
stored at 4°C.

Publication protocol

For comparison of plasma ET-1 between groups, blood samples (about 1ml) were drawn at the time of sacrifice from the left ventricle under general anesthesia using ketamine/xylazine/normal saline cocktail (1ml:0.5ml:8.5ml, 1ml/100g B.W.). Plasma ET-1 level was evaluated using ELISA kits (ab133030, Abcam, Cambridge, UK) according to the manufacturer's instructions.

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Papers

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Manufacturer protocol

Download the product protocol from Abcam for Endothelin 1 ELISA Kit (ab133030) below.

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