Mouse GDNF PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Mice Brain Samples were homogenized in T-PER Tissue Protein Reagent (PI-#78510 ) with Protease and Phosphatase inhibitor cocktail ( PI-78443) and each brain sample’s protein concentration was measured by a BCA kit (Fisher Scientific #23225). Levels of Mouse BDNF and GDNF were measured using a Mouse BDNF and GDNF ELISA Kit from Boster Biological Technology (Fremont, CA. Cat # EK0309 and # EK0935) and followed the Kit instruction and Preparation. The ELISA 96-well plate was read at a Plate Reader (BioTek Synergy) at O.D absorbance at 450nm. The Cytokine concentration was expressed at Unit pg/ml with sample total protein concentration at 1mg/ml.

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Manufacturer protocol

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