GM-CSF Mouse ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Allow the microtiter plates to equilibrate to room temperature before opening the foil bags.	-Run a standard curve with each assay. -Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells. -Do not cover plate with foil.	-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity. -Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days.

Upstream tips
-Allow the microtiter plates to equilibrate to room temperature before opening the foil bags.
Protocol tips
-Run a standard curve with each assay. -Drain residual wash liquid from the wells by efficient aspiration or by decantation followed by tapping the plate forcefully on absorbent paper. Never insert absorbent paper directly into the wells. -Do not cover plate with foil.
Downstream tips
-Once the desired number of strips are removed, immediately reseal the bag and store the plate at 2 to 8°C to maintain plate integrity. -Store both the concentrate and the Working Wash Buffer in the refrigerator. Use the diluted buffer within 14 days.

Publication protocol

The levels of interleukins and growth factors in cell culture supernates mediated by the exposure with glycoconjugates GM-1 and GM-2, respectively, were assayed with Quantikine ELISA® Mouse M-CSF [Cat#MMC00, R&D, USA, minimum detectable dose (MDD) <5 pg/mL], Platinum ELISAs®: Mouse IL-12 (p70) (Cat#BMS616, MDD 4 pg/mL), Mouse GM-CSF (Cat#BMS612, MDD 2 pg/mL), Mouse IL-17(Cat#BMS6001, MDD 1.6 pg/mL), Mouse IL-2(Cat#BMS601, MDD 5.3 pg/mL), and Mouse IL-6 (Cat#BMS603/2, MDD 6.5 pg/mL); Instant ELISAs®: Mouse IL-1β (Cat#BMS600/2INST, MDD 3 pg/mL), Mouse tumor necrosis factor (TNF)-α (Cat#BMS607/2INST, MDD 4 pg/mL), Mouse IL-10 (Cat#BMS614INST, MDD 5.28 pg/mL), all from Affymetrix e-Bioscience, USA, according to the instructions of the manufacturer.

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Manufacturer protocol

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