Q-Plex™ Mouse Cytokine – Inflammation (14-plex)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Allow all reagents to equilibrate to room temperature (20-25°C).	-Allow Substrate A and B to come to room temperature (20-25°C). -Do not allow the plate to dry out between steps. -Do not allow the substrate or SHRP to be exposed to UV light, as this may degrade it.	- Store at room temperature (20-25°C) after mixing.

Upstream tips
-Allow all reagents to equilibrate to room temperature (20-25°C).
Protocol tips
-Allow Substrate A and B to come to room temperature (20-25°C). -Do not allow the plate to dry out between steps. -Do not allow the substrate or SHRP to be exposed to UV light, as this may degrade it.
Downstream tips
- Store at room temperature (20-25°C) after mixing.

Publication protocol

A mouse IgG-specific enzyme-linked immunosorbent assay (ELISA) was performed to assess IgG concentration in serum and cortex using goat anti-mouse IgG Fc-specific antibodies (Jackson ImmunoResearch Laboratories Inc., West Grove, USA) [17]. Cytokine and chemokine concentrations were determined using a multiplex ELISA (Q-plex Mouse Cytokine—Screen (16-plex); Quansys Bioscience, Logan, UT, USA). The following cytokines and chemokines were analyzed: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, tumor necrosis factor (TNF)α, granulocyte-macrophage colony-stimulating factor (GM-CSF), regulated on activated, normal T cell expressed and secreted (RANTES), monocyte chemoattractant protein-1 (MCP-1, also called CCL2), and macrophage inflammatory protein 1α (MIP-1α).

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Manufacturer protocol

Download the product protocol from Quansys Biosciences for Q-Plex™ Mouse Cytokine – Inflammation (14-plex) below.

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