FITC Annexin V Apoptosis Detection Kit I (RUO)

Protocol tips

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	- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Protocol tips
- Treated with FITC Annexin V and PI and incubated for 15 min at RT (25°C) in the dark

Publication protocol

For apoptosis assays, staining with FITC-Annexin V and propidium iodide (PI) was performed using the Annexin V-FITC/PI Apoptosis Detection Kit (BD Biosciences, San Jose, CA) and the apoptosis rates were analyzed by flow cytometry. The Annexin V- and PI+ represented necrotic cells, the Annexin V+ and PI+ represented late apoptotic cell, the Annexin V+ and PI- represented early apoptotic cell, and the Annexin V- and PI- represented normal cells.

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Manufacturer protocol

Download the product protocol from BD Biosciences for FITC Annexin V Apoptosis Detection Kit I (RUO) below.

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