Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
-Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
|
Upstream tips |
-Bring all reagents to room temperature (18-25°C) before use for 30min.
-Prepare fresh standard for each assay. Use within 4 hours and discard after use.
-Centrifuge the sample again after thawing before the assay. |
Protocol tips |
- To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps.
-Once reagents have been
added to the well strips, DO NOT let the strips DRY at any time during the assay.
-TMB Substrate should remain colorless or light blue until added to the plate. Please protect it from light.
-Stop Solution should be added to the plate in the same order as the TMB Substrate. |
Publication protocol
Fourteen hours after LPS (or saline) injection, heme oxygenase-1 (HO-1) was evaluated in the supernatant of homogenized lung tissue using the mouse HO-1 ELISA kit (Cusabio Bio-Tech, Wuhan, China). The homogenates were centrifuged for 5 minutes at 5000 × g at 4°C. The supernatant was diluted 1500-fold for the HO-1 assay.
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