HSP70 ELISA Kit (ab133060)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

For HSP-70 ELISA, hippocampi were snap-frozen in liquid nitrogen and weighed. Samples were homogenized for 15 s using a tissue homogenizer (Omni International, TH2000), followed by the addition of 50 µL of cold (4℃) 1×extraction buffer from the HSP-70 ELISA kit (Abcam, ab133060; Abcam, Cambridge, MA, USA). Following 30-min incubation on ice, samples were sonicated on level 7 with 12 pulses (Sonic Dismembrator 60; Fisher Scientific, Chicago, IL, USA) and centrifuged for 10 min at 21000×g in a 4℃ refrigerated microfuge. The supernatant was diluted 1 : 5 in sample diluent 2 (a component in HSP-70 ELISA kit); 100-µL duplicates were used in the ELISA, and the remaining supernatant was used for protein assays. Excess supernatant was stored at -70℃. The protocol provided with the HSP-70 ELISA kit was followed without any modifications for the quantification of HSP-70 protein levels. A µQuant (Bio-Tek Instruments, Inc., Winooski, UT, USA) was used to measure absorbance at 450 nm, and data were analyzed using Ascent for µQuant.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - HSP70 using HSP70 ELISA Kit (ab133060) from Abcam.

Manufacturer protocol

Download the product protocol from Abcam for HSP70 ELISA Kit (ab133060) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - HSP70 using HSP70 ELISA Kit (ab133060) from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.