Mouse ICAM-1/CD54 Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.

Upstream tips
-Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. The color developed in the wells will turn from blue to yellow upon addition of the Stop Solution.

Publication protocol

IL-6, TNF-α and soluble ICAM-1
protein was quantified from plasma isolated from mice that were fasted overnight using
Quantikine ELISA kits from R&D Systems, according to the manufacturer’s
recommendations.

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Manufacturer protocol

Download the product protocol from R&D Systems for Mouse ICAM-1/CD54 Quantikine ELISA Kit below.

Download manufacturer protocol

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