Mouse ICAM-1 / CD54 PicoKine™ ELISA Kit

ELISA Mouse - ICAM-1/CD54

Experiment
ELISA Mouse - ICAM-1/CD54
Product
Mouse ICAM-1 / CD54 PicoKine™ ELISA Kit from BosterBio
Manufacturer
BosterBio

Protocol tips

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months.
-Avoid multiple freeze-thaw cycles.
-The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment.
-Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature.
-Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

The concentrations of ICAM-1, VCAM-1 and CCL2 in plasma, as well as the supernatants collected from treated RAECs were determined using rat enzyme-linked immunosorbent assay kits (Boster, Wuhan, China) according to the instructions provided by manufacturer. The color absorbance at 450 nm was measured using a Bio-Rad microplate reader.

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Papers

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Manufacturer protocol

Download the product protocol from BosterBio for Mouse ICAM-1 / CD54 PicoKine™ ELISA Kit below.

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