Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.	-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Upstream tips
-Store the unopened kit at 2-8 °C. -Bring all reagents to room temperature before use. -Reconstitute the Human Cytochrome c Standard with deionized or distilled water. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
Protocol tips
-When mixing or reconstituting protein solutions, always avoid foaming. -Substrate Solution should remain colorless until added to the plate. -Keep Substrate solution protected from light. -Stop Solution should be added to the plate in the same order as the Substrate Solution. -Wells that are green in color indicate that the Stop Solution has not mixed thoroughly with the Substrate Solution.

Publication protocol

Serum samples from all animals were obtained via whole blood centrifugation (10 min, 4°C, 3,500 RPM). We used Quantikine Quantitative Sandwich Enzyme Immunoassay ELISA kits for IL-2, IL-4, IL-12, IL-6, IGF-1, IFN-α, IFN-γ, and TNF-α (R&D Systems, Minneapolis, MN, USA) and for leptin and adiponectin (Millipore, St. Charles, MO, USA). All plates were analyzed using a BioRad 680 microplate reader, (Bio-Rad Laboratories, Hercules, CA, USA) at 450 and 570-nm wavelengths, according to the manufacturers’ recommendations.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ELISA Mouse - IGF-I using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit from R&D Systems.

Manufacturer protocol

Download the product protocol from R&D Systems for Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ELISA Mouse - IGF-I using Mouse/Rat IGF-I/IGF-1 Quantikine ELISA Kit from R&D Systems. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.