Mouse IGF1 ELISA Kit (ab100695)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Using specific enzyme-linked immunosorbent assays, systemic and local total IGF1 was measured in serum and liver, respectively; IGF1 was also measured in interstitial fluid of the thymus (obtained by gentle intra-thymic washing) and leptin levels were measured in serum (Abcam Cambridge, UK; Cat.# ab100695 and ab100718, respectively) according to the procedures provided by the manufacturer. Levels were expressed in ng/mL for serum, pg/mL for fluid and pg/mg tissue for the liver. Chemokine levels of soluble ligands such as CCL5 and CXCL12 were measured in interstitial fluid of the thymus using a DuoSet®ELISA development system (R&D Systems, Cat. DY478 and DY460, respectively) according to the manufacturer’s protocols. Levels were expressed in pg/mL.

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Manufacturer protocol

Download the product protocol from Abcam for Mouse IGF1 ELISA Kit (ab100695) below.

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