Mouse IGF-1 PicoKine™ ELISA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.	-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Upstream tips
-Store at 4°C for 6 months, at -20°C for 12 months. -Avoid multiple freeze-thaw cycles. -The standard solutions are best used within 2 hours.
Protocol tips
-The diluted Avidin-Biotin-Peroxidase Complex (ABC) solution should not be prepared more than 1 hour prior to the experiment. -Prepare the substrate solution immediately before use or bring the pre-made substrate to room temperature. -Sodium azide is an inhibitor of HRP. Do not include the azide in buffers or wash solutions if HRP-labeled conjugate is used for detection.

Publication protocol

Blood samples were taken from the beating heart of anesthesized mice. Serum activities of ASAT, ALAT, and GLDH were measured with an automated analyser (Roche modular) using standardized assays (Roche, Mannheim, Germany). Blood glucose levels were determined using a Blood Glucose Meter (Freestyle Mini, Abbot, Wiesbaden).

Insulin was detected in serum with an Elisa- Kit from DRG Instruments (Marburg, Germany) (EIA 3439). IGF-I, IGFBP-1 and GH, were detected in serum with Elisa-Kits from Mediagnost (Reutlingen, Germany and Cell Applications (San Diego, USA).

To determine IGF-I and IGFBP-I in cell culture supernatant we used Elisa-Kits from Boster Biological Technology (Fremont, CA, USA).

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Manufacturer protocol

Download the product protocol from BosterBio for Mouse IGF-1 PicoKine™ ELISA Kit below.

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