Mouse IL-1 beta ELISA Kit (ab100704)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.	-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.	-Unused well strips should be returned to the plate packet and stored at 4°C.

Upstream tips
-Equilibrate all reagents to room temperature (18-25°C) prior to use.
Protocol tips
-Avoid foaming or bubbles when mixing or reconstituting components. -Ensure plates are properly sealed or covered during incubation steps. -Complete removal of all solutions and buffers during wash steps is necessary to minimize background.
Downstream tips
-Unused well strips should be returned to the plate packet and stored at 4°C.

Publication protocol

Serum levels of cytokines interleukin 6 (IL-6; no. ab46100), IL-1β (no. ab100704), and tumor necrosis factor alpha (TNF-α; no. ab100747) were determined with enzyme-linked immunoassay (ELISA) kits (Abcam, Cambridge, MA, USA).

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Manufacturer protocol

Download the product protocol from Abcam for Mouse IL-1 beta ELISA Kit (ab100704) below.

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